The shows airing during this period set the stage for those to come in 2005 to 2009, the latter part of the decade. However, they appeared more frequently in anime series such as Gravitation, Kino's Journey, Kannazuki no Miko, and My-HiME. From 2000 to 2004, such characters appeared prominently in Queer Duck, Drawn Together, and The Venture Bros. The number of animated series with LGBTQ characters increased from the previous decade.

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